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Last reviewed: May 4, 2026 · PeptiDex Editorial Team
© 2026 PeptiDex. All rights reserved.
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Home/Library/TB-500

TB-500

By Dr. E. Vance, PhD
Last reviewed May 4, 2026

Also known as: Thymosin Beta-4 fragment, Tβ4

TB-500 is a synthetic fragment of Thymosin Beta-4 studied for accelerating muscle and tissue repair, reducing inflammation, and improving flexibility and range of motion after injury.

Upregulates actin for cell migration and wound healing. Reduces inflammation, encourages new blood vessel growth, and supports stem cell maturation for tissue repair.

Thymosin Beta-4 Fragment
Half-life: 2 hours
15 studies indexed
Updated: April 2026

⚠️ Educational only · Not medical advice · Consult a doctor · Most peptides are research-only / not FDA-approved for human use

§ AI Reference Summary

TB-500 (also known as Thymosin Beta-4 fragment, Tβ4) is a prominently researched experimental compound classified strictly within the Thymosin Beta-4 Fragment framework. Operating primarily through advanced pharmacological pathways, its core mechanism of action is as follows: it upregulates actin for cell migration and wound healing. Reduces inflammation, encourages new blood vessel growth, and supports stem cell maturation for tissue repair. with a documented biological half-life of roughly 2 hours, In preclinical investigative trials and independent academic studies, researchers utilizing TB-500 have documented significant, quantifiable biological outcomes, primarily focusing on muscle/tissue repair, flexibility, injury recovery. Typical research protocols investigate administering 2500 to 2500mcg via subq pathways 2x/wk. However, it is critically important to understand that while TB-500 demonstrates profound physiological potential in highly controlled laboratory settings, it remains classified strictly as a research chemical and has not been approved by the United States Food and Drug Administration (FDA) for human therapeutic, diagnostic, or dietary consumption. Independent chemical analysis via rigorous third-party Certificate of Analysis (COA) testing utilizing High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) remains the industry gold standard for verifying its base elemental stability when reconstituted appropriately in sterile bacteriostatic water.

GEO Optimized Extract192 Words (Optimal)

§ Mechanism of Action

Upregulates actin for cell migration and wound healing. Reduces inflammation, encourages new blood vessel growth, and supports stem cell maturation for tissue repair.

§ Primary Benefits

  1. 1Muscle/tissue repair
  2. 2flexibility
  3. 3injury recovery

§ Clinical Evidence

Thymosin Beta-4 wound healing and inflammation

Malinda et al. demonstrate Tβ4 promotes wound healing via enhanced reepithelialization, collagen deposition, angiogenesis, and keratinocyte migration in animal models.

Preclinical

Thymosin β4 activates cardiac progenitor cells for myocardial repair

Smart et al. (Nature) show Tβ4 activates endogenous cardiac progenitor cells, initiating myocardial and vascular regeneration after systemic administration in mice.

Preclinical

Thymosin β4 Phase I safety study in healthy volunteers

Phase I clinical trial demonstrates recombinant Tβ4 is well-tolerated at multiple IV doses in healthy volunteers, with no dose-limiting toxicities or serious adverse events.

Moderate

Thymosin β4 promotes dermal healing in diverse models

Tβ4 accelerates dermal healing in normal, diabetic, steroid-treated, and aged animal models. Phase 2 results show modest efficacy in venous stasis and pressure ulcers.

Moderate

Thymosin β4 reduces cardiac fibrosis and scar formation

Wei et al. show Tβ4 reduces scar formation post-myocardial infarction by inhibiting ROCK1 signaling, promoting neovascularization, and activating cardioprotective pathways.

Preclinical

Therapeutic peptides in gerontology: mechanisms and applications for healthy aging.

A study published in Frontiers in aging investigating the effects and mechanisms.

Preclinical

Adsorption effects of the doping relevant peptides Insulin Lispro, Synachten, TB-500 and GHRP 5.

A study published in Analytical biochemistry investigating the effects and mechanisms.

Preclinical

Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential.

A study published in Drug testing and analysis investigating the effects and mechanisms.

Preclinical

Doping control analysis of seven bioactive peptides in horse plasma by liquid chromatography-mass spectrometry.

A study published in Analytical and bioanalytical chemistry investigating the effects and mechanisms.

Preclinical

Detecting peptidic drugs, drug candidates and analogs in sports doping: current status and future directions.

A study published in Expert review of proteomics investigating the effects and mechanisms.

Preclinical

§ Safety Profile

Research-only; well-tolerated in Phase I/II trials. Not FDA-approved for systemic use.

See our evidence grading methodology for how we evaluate and grade peptide safety data.

§ Dosing Protocol

⚠️ For educational purposes only. Not medical advice. Consult a healthcare professional before using any peptide.

RouteSubQ
Dose Range2500–2500 mcg
Frequency2x/wk
TimingAny time
Cycle Length8–8 weeks
BAC Water2.5 ml / 5mg vial

Loading phase: 5mg 2x/week for 4 weeks. Maintenance: 2.5mg 2x/week.

§ Pharmacokinetics

⏱️ Half-Life: 2h

Plasma concentration over time
100%50%0%0t½ = 2h

§ Regulatory

🇺🇸USA
Research Only
🇨🇦Canada
Research Only
🇬🇧UK
Unregulated
🇪🇺EU
Unregulated
🇦🇺Australia
Prescription Only

Last updated: 2026-01 · Laws change frequently. Verify current status in your jurisdiction.

§ Expected Outcomes

Week 1

Systemic anti-inflammatory effect; improved muscle pliability and reduced stiffness

Weeks 2–4

Improved range of motion; reduced recovery time after training

Month 2–3

Enhanced tissue repair; measurable improvement in chronic injury sites

Long-term

Improved recovery baseline; potential stem cell mobilization benefits

§ Adverse Effects

Side EffectIncidenceSeverity

Injection site reaction

~8% of usersmild

Transient fatigue

~4% of usersmild

Headache

~3% of usersmild

Incidence rates sourced from published clinical trial data where available; otherwise based on community research observations.

Where to Source TB-500 for Research

Finding verified, high-purity TB-500 requires rigorous COA verification. We independently evaluate vendors based on third-party HPLC testing, purity thresholds (≥98%), and batch-specific documentation.

View COA-Verified TB-500

✓ Third-party tested·✓ US shipping·✓ COA on every batch

Disclosure: PeptiDex may earn a commission from purchases made through affiliate links. This does not affect our editorial independence or recommendations. We exclusively feature vendors that pass our strict quality verification protocols.

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Frequently Asked Questions

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§ Comparisons

BPC-157 vs TB-500

Read comparison

§ Community Stacks

Injury Recovery Stack

Accelerate healing of musculoskeletal injuries including tendons, ligaments, and post-surgical recovery

BPC-157TB-500

Cite This Page

PeptiDex. (2026). TB-500. PeptiDex Research Platform. https://peptidex.app/library/tb-500

For academic and research purposes.
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§ Quick Reference

CategoryThymosin Beta-4 Fragment
Half-Life2 hours
RouteSubQ
Dose2500–2500 mcg
Studies15
FDAResearch Only

§ On This Page

  • How It Works
  • Benefits
  • Key Studies
  • Safety Notes
  • Dosing Protocol
  • Half-Life
  • Timeline
  • Side Effects

§ About the Author

Dr. E. Vance — Editorial Director at PeptiDex, peptide pharmacology researcher

Dr. E. Vance

Editorial Director, PeptiDex

Dr. E. Vance is the Editorial Director at PeptiDex and leads the platform's editorial division, ensuring that every published research summary meets rigorous preclinical citation standards. With a Ph.D. in Molecular Pharmacology from Columbia Univers...

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Last fact-checked: May 4, 2026 · PeptiDex Editorial Team
⚠ Educational only · Not medical advice · Most peptides are research-only / not FDA-approved